Bio-Rad offers a complete workflow solution for protein detection and quantitation featuring proprietary stain-free imaging technology, primary and secondary antibodies, as well as fluorescent and chemiluminescent imaging systems. “The Bio-Rad Western Blot Learning Center extends our support to researchers, offering insights and techniques on blotting from our experts,” he added.Įlectrophoresis and western blotting are standard research methods used to characterize proteins. The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use. “We’re delighted to share our experiences with researchers to help them optimize their experiments and achieve more reliable results,” said Ben Wang, Bio-Rad Global Product Manager of Electrophoresis and Western Blotting Products, Life Science Group. The center offers comprehensive information on the science of western blotting, best practices, tips and techniques, as well as guidance on how to troubleshoot experiments. The Bio-Rad Western Blot Learning Center, available on, was created to provide researchers an online platform to learn about western blotting, leveraging Bio-Rad’s experience and expertise in western blotting as well as protein electrophoresis that spans decades. Recommended volume per well for 1.0 mm mini gelġ0 μL for electrophoresis, 5 μL for blottingĥ μL for electrophoresis, 2.Bio-Rad Laboratories today announced the launch of the Bio-Rad Western Blotting Learning Center, an online library that provides comprehensive resources, information, and guidance to help researchers improve their experimental western blotting (or immunoblotting) approaches to obtain high-quality data. ~160, 120, 80, 60, 50, 40, 30, 20, 15, 10 kDaĮach protein standard contains a 6X His-tag enabling the detection with the InVision His-tag In-gel StainĬolorimetric or methods that detect phosphorylated proteins such as Pro-Q Diamond phosphoprotein gel stainsĬolorimetric or methods that detect glycosylated proteins such as Pro-Q Glycoprotein stain kits Peppermint Stick Phosphoprotein Molecular Weight StandardsĬand圜ane Glycoprotein Molecular Weight Standards Tagged- proteins in the 10 -250 kDa range contain a Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequenceĭirect visualization can be achieved through standard staining protocols (e.g., Coomassie, etc.) Thermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color protein standard containing 10 prestained proteins (10 to 260kDa) for use in gel electrophoresis and Western blotting.Features of Spectra Multicolor Broad Range Pr. Rinse the blot with 15 ml TBST at RT for 5 min. Incubate the blot in the primary antibody and blocking buffer solution at 4☌ overnight with gentle agitation. Tagged-proteins contain an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequence SuperSignal West Femto Maximum Sensitivity Substrate. Dilute the primary antibody 1:1,000 in 10 ml blocking buffer. Compatible with chemiluminescent substrates and fluorescent secondary antibodies. PageRuler Unstained Broad Range Protein LadderĪccurate estimation across a broader range Western blot confirmationdetect the two unstained proteins (30 kDa and 80 kDa) using the detection system for the target protein Applications Western blotting: detection of the two unstained bands via the detection method used for the target protein. Recommended Volume per well for 1.0 mm gel (μL)Ĭolorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands) Precision Plus Protein All Blue Prestained Protein Standards 1610373 Bio-Rad. Stains to visualize and quantify protein bands on gels and blots: Coomassie stains, highly sensitive fluorescent stains, silver stains, negative stains, and total protein blot. Visible monitoring of gel separation and transfer efficiency Western blotting products include protein transfer systems, blotting membranes, filter paper, premixed blotting buffers and reagents, and detection kits. Spectra Multicolor Broad Range Protein Ladderīest multicolor prestained ladder for routine applicationsĤ colors for improved visualization during separation and transferĪnalysis of high molecular weight proteins
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